tloaelo ea oligo process workstation

tloaelo ea oligo process workstation,
oligo process thepa ea thepa,

Karolo ea Elution

1. Mola oa likoti tse 8 tsa ente ea metsi, tse ka kenngoa ka nako e le 'ngoe,' me metsi a bohlokoa a ka laoloa ka bomong.
2. 5 maemo a botlolo ea reagent, botlolo e 'ngoe le e' ngoe e na le botlolo ea 4L ea reagent, ke hore, e ka lumellana le mefuta e 5 ea li-reagents.
3. Sesebelisoa se khannoa ke mokelikeli o motle oa khatello, o loketseng le o feto-fetohang ho laola sekhahla sa phallo ea metsi.
4. Thepa e na le boemo ba poleiti ea seliba sa 96, 'me mofuta oa poleiti o hlalosoa ke moreki.
5. Letlapa le tebileng la liliba tse 96 le ka behoa ka tlas'a poleiti ho bokella mokelikeli, kapa le ka hokeloa ka ho toba pompong ho ntša metsi a litšila.
6. Karolong ea software, o ka khetha mofuta oa reagent le palo ea reagent ho eketsa sampuli, nako e ka behoa, mochine oa khatello o ka behoa, nako ea khatello e ka laoloa, 'me mehato e ka behoa ho matha ka ho sa feleng.Mefuta e 2 ea diresepe.

Karolo ea tlhoekiso

1. Thepa e ka tlatsa litšiea tse 96 c18 ka nako e le 'ngoe.
2. Thepa e tloaelehileng e na le likou tse hlano tsa reagent, centrifuge tube holder, 'me motho ea nang le eona a ka jara boholo ba 96 primer centrifuge tubes.
3. Thepa e amohela tsamaiso e ikemetseng ea li-axis tse tharo, ka ho nepahala ho phahameng le ho pheta-pheta hantle.
4. Thepa e amohela mofuta oa filthara ea ho monya.
5. Litlhophiso tsa software lia tenyetseha ebile li bonolo ho sebetsa.
6. Hlooho ea ho hula e na le sesebelisoa se utloang boemo ba mokelikeli se khonang ho lemoha bophahamo ba boemo ba mokelikeli.

Karolo ea pipeting

1. Letlapa la seliba sa 96 ho tube ea centrifuge.
2. Tlhahiso e ikemetseng ea pipette: primer e 'ngoe le e' ngoe e ka aroloa ka lihlopha tse 2 ka ho fetisisa.
3. X, Y, Z motsamao oa axis.
4. Mefuta e mengata ea molumo oa ho hula (5-200ul).
5. Ha u aroloa ka li-tubes tse ngata.
6. Litlhoko tsa nako.
7. E ka amahanngoa le template ea tekanyo, Ts'ebetso ea potlako ea phoso, ho na le mosebetsi oa ho emisa bohareng o Bofubelu o bontšang hore molumo o feletseng ha oa lekana 'me o hloka ho kopanngoa.
Ho na le malebela a khubelu, a mosehla le a botala bakeng sa ho bala molumo: mosehla o bolela hore molumo o fokotsehile, o ka fetola molumo ka letsoho, ha ho hlokahale hore o kopane, botala bo bolela ntho e tloaelehileng.
8. Tlhahisoleseding e kang nomoro ea kholomo, palo ea li-tubes, le molumo oa tube e 'ngoe le e' ngoe li bontšoa ha ho tlatsa sampuli ka 'ngoe.Boemo bo bonts'a hore li-primers li arotsoe 'me ha lia aroloa, tokiso ea boemo bo itekanetseng pele ho phano e 'ngoe le e 'ngoe.

tloaelo ea oligo process workstation ea oligo process aupport lisebelisoa, e na le ts'ebetso ea Pepetting, ho kopanya, ho sisinyeha, ho futhumatsa joalo-joalo.