Maraba a molek'hule bakeng sa phosphoramidite le li-reagents

Kopo:

The Molecular Trap e sebelisetsoa ho adsorb trace water in the reagents le amidite, qalong e ne e etselitsoe ho qaptjoa ha oligonucleotides.E bonolo, ha e na lerōle ebile ha e na flannel.E ka ekeletsoa mefuteng e fapaneng ea li-solvents le litharollo tsa manyolo ho tlosa palo ea metsi.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Tlhaloso ea Sehlahisoa

Litekong tsa ABI le MilliGen/PerSeptive DNA synthesis, sub-sieve pack e ka boloka metsi a ka har'a libotlolo tsa nitrile le activator ka tlase ho 10 ppm, ntle le ho tšoenyeha ka valve kapa valve ea throttle e arotsoe ka sub-sieve.Lerole kapa lesela le ka mokotleng oa ho paka le koalehile.E ka sebelisoa ka kotloloho bakeng sa ho nosetsa 500 ml, 1L, 2 L le libotlolo tse ling tsa solvent, 'me litlhaloso tsa pakete ea sub-sieve le tsona li ka etsoa.

Lintlha tse latelang li bonts'a phello e matla ea ho tlosa metsi ea 10 g ea leraba la molek'hule ka 500ml, 1L, 4L disampole tsa nitrile tse nang le metsi a fapaneng.

Bapisa le sefi sa import molecular trap

500 ml 197 ppm ACN
Nako(h)	0	24	48	72	96
HonyaBio	197	33	16.5	6.5	6
Linaha Tse Ling	197	43	27	15	15

1 L 143 ppm ACN
Nako(h)	0	24	48	72	96
HonyaBio	143	48	32	20	15
Linaha Tse Ling	142	47	36	23	15

4 L 141 ppm ACN
Nako(h)	0	24	48	72	96
HonyaBio	141	95	94	84	73
Linaha Tse Ling	141	96	95	85	72

Litaelo le tšebeliso

Sefi sa molek'hule se na le vacuum-packed, 'me se hloka ho buloa leha se sebelisoa,' me tiiso ea botlolo ea reagent e tlameha ho netefatsoa nakong ea tšebeliso.
2g sieve bakeng sa 24 h e ka fokotsa metsi a 165 ppm ho 500 ml nitrile ho ea ho 105 ppm.
5 g sieve bakeng sa 24 h e ka fokotsa metsi a 172 ppm ho 500 ml nitrile ho ea ho 58 ppm.
10 g sieve 24 h E ka fokotsa metsi a 166 ppm ka 1 L nitrile ho ea ho 68 ppm.
20 g sub-sieve e ka fokotsa metsi a 162 ppm ho 4 L nitrile ho ea ho 109 ppm bakeng sa 24 h.

Re khothaletsa 2 g subsieve bakeng sa libotlolo tsa reagent tsa 50-250ml, 5g bakeng sa libotlolo tsa reagent tse 250-500ml, 10g bakeng sa libotlolo tsa reagent tsa 500-1000ml, le 20g bakeng sa libotlolo tsa reagent tse 1000-2000ml.

Tiisetso

Sephutheloana se seng le se seng sa skrine se senyenyane se tlas'a taolo e tiileng ea boleng nakong ea ts'ebetso ea tlhahiso.'Me hoa hlokahala hore bareki ba hlahlobe sephutheloana pele ba se sebelisa:

Ntlha ea pele, netefatsa hore vacuum ea ho paka e ntse e tiile.Ho lutla ha vacuum efe kapa efe kapa ho kena moeeng ho tla fokotsa ts'ebetso ea sehlahisoa.

Ea bobeli, e-ba hlokolosi ha u bula sephutheloana ho thibela filimi ea sephutheloana se senyenyane hore e se ke ea ngolisoa, 'me u hlahlobe hore na filimi e Ho na le ho lutla.

Kalafo ka mor'a tšebeliso

Ha e chefo kapa e kotsi, li tla silafatsoa ke lik'hemik'hale le lihlapolli tseo li kopanang le tsona 'me li lokela ho lahloa e le litšila tse silafetseng ka mor'a ho sebelisoa.

Mohlala le tšebeliso

Re kile ra fana ka mekotla ea sub-sieve ka boholo ba 2 g, 5 g, 10 g, le 20 g., 'me litlhaloso tse ling li ka etsoa ho latela litlhoko tsa bareki.

Sefi sa molek'hule se loketse ho pepesehela nitrile ka nako e telele, 'me se ka boela sa sebelisoa bakeng sa acetic acid, ether, acetic ether, butyl acetate, joala, isopropanol, methanol, butanol, Phenol, pyridine, hydrochloric acid, nitric acid, phosphoric acid. , sulfuric acid, methyl chloride, nitrogen methyl imidazole, joalo-joalo.

E ka 'na ea se ke ea tšoaneleha bakeng sa ho pepeseha ha nako e telele ho tetrahydrofuran, toluene, methyl formamide (DMF), methyl methyl amide (DMAc), N-methylpyrrolidone (NMP) le tharollo e meng.