Molao-motheo oa Oligo Synthesizer
Likarolong tsa lipatlisiso tsa baeloji ea limolek'hule le liphatsa tsa lefutso, bokhoni ba ho kopanya DNA bo phetha karolo ea bohlokoa.DNA synthesis e kenyelletsa tlhahiso ea maiketsetso ea limolek'hule tsa DNA ka ho hlophisa li-nucleotide ka tatellano e itseng.Ho finyella sena, bo-rasaense ba itšetlehile ka sesebelisoa se matla se tsejoang e le oligonucleotide synthesizer, eo hape e tsejoang e le DNA synthesizer.
Oligonucleotide synthesizer ke sesebelisoa se rarahaneng se iketsetsang limolek'hule tse khutšoane tsa DNA tse bitsoang oligonucleotides.Likhoele tsena tse khutšoane tsa DNA hangata li na le li-nucleotide tse 10 ho isa ho tse 100 ka bolelele 'me ke litene tsa bohlokoa tsa ho aha lits'ebetsong tse fapaneng tse kenyelletsang polymerase chain reaction (PCR), gene synthesis, genetic engineering le DNA sequencing.
Li-synthesizer tsa oligonucleotide li sebetsa ho latela molao-motheo oa mokhoa o tsejoang e lemokhahlelo o tiileng.Mokhoa ona o ile oa qalisoa ka lekhetlo la pele ke mohapi oa Nobel Dr. Marvin Caruthers lilemong tsa bo-1970 'me o 'nile oa hloekisoa ho theosa le lilemo ho ntlafatsa motsoako oa tatellano ea DNA.Oligonucleotide synthesis e etsoa ka ho eketsoa ka mohato oa masala a nucleotide ho 5'-terminus ea ketane e ntseng e hola ho fihlela tatellano e lakatsehang e kopantsoe.Keketso e 'ngoe le e' ngoe e bitsoa "synthesis cycle" mme e na le karabelo ea lik'hemik'hale tse 'ne:
Mohato oa 1: Ho thibela (detritylation) -----------Mohato oa 2: Ho kopanya---------Mohato oa 3: Capping------------Mohato oa 4: Oxidation
Ts'ebetso ena e phetoa bakeng sa nucleotide ka 'ngoe ho fihlela tatellano e lakatsehang e fumanoa.Bakeng sa li-oligonucleotide tse telele, potoloho ena e ka 'na ea hloka ho phetoa ka makhetlo a' maloa ho kopanya tatellano eohle. Bokhoni ba ho laola ka nepo mohato o mong le o mong oa potoloho ea motsoako bo bohlokoa ho oligonucleotide synthesizer.Li-reagents tse sebelisoang, joalo ka li-nucleotide le li-activator, li hloka ho ba tsa boleng bo holimo ho netefatsa synthesis e nepahetseng le e sebetsang hantle.Ntle le moo, li-synthesizer li hloka taolo e nepahetseng ea mocheso o phahameng le maemo a mang a tikoloho ho khothaletsa karabelo e lakatsehang ea ho kopanya le ho thibela maikutlo a sa batleheng.
Hang ha oligonucleotide e se e entsoe ka botlalo, hangata e khaoha ho tsoa ho ts'ehetso e tiileng ebe e hloekisoa ho tlosa lihlopha tse setseng tse sireletsang kapa litšila.Li-oligonucleotide tse hloekisitsoeng li se li loketse ho sebelisoa ka tlase.
Tsoelo-pele ea thekenoloji e nolofalelitse nts'etsopele ea li-oligonucleotide synthesized synthesizer tse khonang ho kopanya makholo kapa esita le likete tsa oligonucleotide.Lisebelisoa tsena li sebelisa theknoloji e thehiloeng ho microarray-based synthesis, e nolofalletsang bafuputsi ho hlahisa lilaebrari tse kholo tsa oligonucleotide ka potlako bakeng sa merero e fapaneng ea lipatlisiso.
Ka bokhutšoanyane, melao-motheo e ka morao ea oligonucleotide synthesizer e pota-potile mekhoa ea ho kopanya e tiileng, e kenyelletsang ho kenyelletsoa ha li-nucleotide ka mohato o tiileng.Taolo e nepahetseng ea potoloho ea li-synthesis le li-reagents tsa boleng bo holimo li bohlokoa bakeng sa motsoako o nepahetseng le o sebetsang hantle.Li-Oligo synthesizer li phetha karolo ea bohlokoa lipatlisisong tsa DNA, tse nolofalletsang bo-rasaense ho hlahisa li-oligonucleotide tse tloaelehileng bakeng sa mekhoa e fapaneng ea ts'ebeliso, e leng se tlatsetsang tsoelo-peleng ea theknoloji ea baeloji le lipatlisiso tsa lefutso.
Nako ea poso: Aug-01-2023