Lisebelisoa tsa Oligo Synthesis
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Likotlolo tsa botlolo bakeng sa Phosphoramidite le Reagents
E sebelisoa bakeng sa botlolo ea Phosphoramidite le botlolo ea Oligo synthesis reagent, ho na le mefuta e sa tšoaneng ea li-caps tse peli, u ka khetha ho ea ka litlhoko.
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Maraba a molek'hule bakeng sa phosphoramidite le li-reagents
The Molecular Trap e sebelisetsoa ho adsorb trace water in the reagents le amidite, qalong e ne e etselitsoe ho qaptjoa ha oligonucleotides.E bonolo, ha e na lerōle ebile ha e na flannel.E ka ekeletsoa mefuteng e fapaneng ea li-solvents le litharollo tsa manyolo ho tlosa palo ea metsi.
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Lipoleiti tsa Sieve le Lisebelisoa tsa Oligo Synthesis
Sieve Plate le filthara li tšeloa ka li-olefin tse phahameng haholo tse nang le boima ba molek'hule bo fetang milione e le 'ngoe.E na le khanyetso e ntle ea lik'hemik'hale le hydrophobicity, 'me e bolokehile ebile ha e na chefo.
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CPG Frit kholomo e nang le boholo bo fapaneng
Moloko oa bobeli oa motsoako oa bokahohle o kopanya CPG le thekenoloji ea likaroloana tse nyenyane le thekenoloji ea carrier ea bokahohle, ho kopanya CPG le lipoleiti tsa sieve tse ka holimo le tse tlaase ka kakaretso.Ka ho ntlafatsa bolelele le bophara, re ka fokotsa tšebeliso ea li-reactants le li-detergents le ho fokotsa motsoako.Ho khangoa ke metsi ho etsa hore ho se be le metsi hantle.
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Universal Column bakeng sa li-Oligo Synthesizer tse fapaneng
Moloko oa pele oa motsoako oa motsoako o tletse CPG e tsamaisang karolo e tiileng ka har'a tube ea kholomo 'me e tsitsitsoe ke lipoleiti tsa sieve tse ka holimo le tse tlaase.E na le phallo e phahameng ea synthesis 'me e bonolo ho e kopanya, e loketse ho kopanngoa ha li-primers tsa ketane e khutšoanyane.
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394 Column Synthesis bakeng sa Oligo Synthesizer
Kholomo ena e loketse ABI, K&A synthesizer, haeba u na le lisebelisoa tsena, u ka khetha kholomo ena, re ka fana ka sehlahisoa se theko e tlase le ts'ebeletso e ntle ea kamora ho rekisa.
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Kholomo e kholo ea Synthesis ka theko e ntle ka ho fetisisa
CPG e pakang e nang le Universal linker e ntlafatsa sekhahla sa phallo ea poleiti ea sieve, e loketseng motsoako o moholo ebile e lumellana hantle.E na le mefuta e fapaneng ea boholo, haholo-holo e loketseng synthesis e kholo.
